Ratiometric Analysis of Fura Red by Flow Cytometry: A Technique for Monitoring Intracellular Calcium Flux in Primary Cell Subsets

Open Access

Peer-reviewed

Research Article

Emily R. Wendt,

Affiliation Nuffield Department of Clinical Medicine, Experimental Medicine Division, Translational Gastroenterology Unit, University of Oxford, Oxford, United Kingdom
⨯
Helen Ferry,

Affiliation Nuffield Department of Clinical Medicine, Experimental Medicine Division, Translational Gastroenterology Unit, University of Oxford, Oxford, United Kingdom
⨯
David R. Greaves,

Affiliation Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
⨯
Satish Keshav

* E-mail: satish.keshav@ndm.ox.ac.uk

Affiliation Nuffield Department of Clinical Medicine, Experimental Medicine Division, Translational Gastroenterology Unit, University of Oxford, Oxford, United Kingdom
⨯

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T cells
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Calcium signaling
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Lasers
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Chemokines
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Cell staining
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Flow cytometry
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Drug therapy
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Monocytes
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