Homologous Expression of the Caldicellulosiruptor bescii CelA Reveals that the Extracellular Protein Is Glycosylated

Daehwan Chung; Jenna Young; Yannick J. Bomble; Todd A. Vander Wall; Joseph Groom; Michael E. Himmel; Janet Westpheling

doi:10.1371/journal.pone.0119508

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Peer-reviewed

Research Article

Homologous Expression of the Caldicellulosiruptor bescii CelA Reveals that the Extracellular Protein Is Glycosylated

Daehwan Chung ,

Contributed equally to this work with: Daehwan Chung, Jenna Young

Affiliations Department of Genetics, University of Georgia, Athens, Georgia, United States of America, The BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯
Jenna Young ,

Contributed equally to this work with: Daehwan Chung, Jenna Young

Affiliations Department of Genetics, University of Georgia, Athens, Georgia, United States of America, The BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯
Yannick J. Bomble,

Affiliations Biosciences Center, National Renewable Energy Laboratory, Golden, Colorado, United States of America, The BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯
Todd A. Vander Wall,

Affiliations Biosciences Center, National Renewable Energy Laboratory, Golden, Colorado, United States of America, The BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯
Joseph Groom,

Affiliations Department of Genetics, University of Georgia, Athens, Georgia, United States of America, The BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯
Michael E. Himmel,

Affiliations Biosciences Center, National Renewable Energy Laboratory, Golden, Colorado, United States of America, The BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯
Janet Westpheling

* E-mail: janwest@uga.edu

Affiliations Department of Genetics, University of Georgia, Athens, Georgia, United States of America, The BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America
⨯

Homologous Expression of the Caldicellulosiruptor bescii CelA Reveals that the Extracellular Protein Is Glycosylated

Daehwan Chung,
Jenna Young,
Yannick J. Bomble,
Todd A. Vander Wall,
Joseph Groom,
Michael E. Himmel,
Janet Westpheling

Published: March 23, 2015
https://doi.org/10.1371/journal.pone.0119508

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Glycosylation
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Signal peptides
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Signal amplification
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Cellulases
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Glycoproteins
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Plasmid construction
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Protein expression
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