Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli

Open Access

Peer-reviewed

Research Article

Ana Paula Jacobus,

Affiliation Department of Biological Sciences, University of São Paulo, Luiz de Queiroz College of Agriculture, Piracicaba, São Paulo, Brazil
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Jeferson Gross

* E-mail: jgross@usp.br

Affiliation Department of Biological Sciences, University of São Paulo, Luiz de Queiroz College of Agriculture, Piracicaba, São Paulo, Brazil
⨯

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Polymerase chain reaction
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Cloning
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Plasmid construction
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Homologous recombination
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Vector cloning
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DNA cloning
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Plasmid vectors
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Stoichiometry
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