Optimization of Heavy Chain and Light Chain Signal Peptides for High Level Expression of Therapeutic Antibodies in CHO Cells

Ryan Haryadi; Steven Ho; Yee Jiun Kok; Helen X. Pu; Lu Zheng; Natasha A. Pereira; Bin Li; Xuezhi Bi; Lin-Tang Goh; Yuansheng Yang; Zhiwei Song

doi:10.1371/journal.pone.0116878

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Open Access

Peer-reviewed

Research Article

Optimization of Heavy Chain and Light Chain Signal Peptides for High Level Expression of Therapeutic Antibodies in CHO Cells

Ryan Haryadi,

Affiliation Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
⨯
Steven Ho,

Affiliation Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
⨯
Yee Jiun Kok,

Affiliation Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
⨯
Helen X. Pu,

Affiliation Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
⨯
Lu Zheng,

Affiliation Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
⨯
Natasha A. Pereira,

Affiliation Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
⨯
Bin Li,

Affiliation Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
⨯
Xuezhi Bi,

Affiliation Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
⨯
Lin-Tang Goh,

Affiliation Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
⨯
Yuansheng Yang,

Affiliation Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
⨯
Zhiwei Song

* E-mail: song_zhiwei@bti.a-star.edu.sg

Affiliation Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
⨯

Optimization of Heavy Chain and Light Chain Signal Peptides for High Level Expression of Therapeutic Antibodies in CHO Cells

Ryan Haryadi,
Steven Ho,
Yee Jiun Kok,
Helen X. Pu,
Lu Zheng,
Natasha A. Pereira,
Bin Li,
Xuezhi Bi,
Lin-Tang Goh,
Yuansheng Yang

Published: February 23, 2015
https://doi.org/10.1371/journal.pone.0116878

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Signal peptides
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Enzyme-linked immunoassays
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Transfection
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Antibody production
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Antibody therapy
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CHO cells
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Endoplasmic reticulum
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