Detection and Quantification of Microparticles from Different Cellular Lineages Using Flow Cytometry. Evaluation of the Impact of Secreted Phospholipase A2 on Microparticle Assessment

Matthieu Rousseau; Clemence Belleannee; Anne-Claire Duchez; Nathalie Cloutier; Tania Levesque; Frederic Jacques; Jean Perron; Peter A. Nigrovic; Melanie Dieude; Marie-Josee Hebert; Michael H. Gelb; Eric Boilard

doi:10.1371/journal.pone.0116812

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Open Access

Peer-reviewed

Research Article

Detection and Quantification of Microparticles from Different Cellular Lineages Using Flow Cytometry. Evaluation of the Impact of Secreted Phospholipase A₂ on Microparticle Assessment

Matthieu Rousseau,

Affiliation Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec, Faculté de Médecine de l’Université Laval, Québec, QC, Canada
⨯
Clemence Belleannee,

Affiliation Centre de Recherche du CHUQ and Département d’Obstétrique-Gynécologie, Faculté de Médecine, Université Laval, Québec, QC, Canada
⨯
Anne-Claire Duchez,

Affiliation Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec, Faculté de Médecine de l’Université Laval, Québec, QC, Canada
⨯
Nathalie Cloutier,

Affiliation Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec, Faculté de Médecine de l’Université Laval, Québec, QC, Canada
⨯
Tania Levesque,

Affiliation Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec, Faculté de Médecine de l’Université Laval, Québec, QC, Canada
⨯
Frederic Jacques,

Affiliation Centre Hospitalier Universitaire de Québec, Québec, Canada
⨯
Jean Perron,

Affiliation Centre Hospitalier Universitaire de Québec, Québec, Canada
⨯
Peter A. Nigrovic,

Affiliations Division of Rheumatology, Immunology and Allergy, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, United States of America, Division of Immunology, Boston Children’s Hospital, Harvard Medical School, Boston, MA, United States of America
⨯
Melanie Dieude,

Affiliation Centre hospitalier de l’Université de Montréal (CRCHUM), Montréal, QC, Canada
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Marie-Josee Hebert,

Affiliation Centre hospitalier de l’Université de Montréal (CRCHUM), Montréal, QC, Canada
⨯
Michael H. Gelb,

Affiliation Department of Chemistry, University of Washington, Seattle, WA, United States of America
⨯
Eric Boilard

* E-mail: eric.boilard@crchuq.ulaval.ca

Affiliation Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier Universitaire de Québec, Faculté de Médecine de l’Université Laval, Québec, QC, Canada
⨯

Detection and Quantification of Microparticles from Different Cellular Lineages Using Flow Cytometry. Evaluation of the Impact of Secreted Phospholipase A₂ on Microparticle Assessment

Matthieu Rousseau,
Clemence Belleannee,
Anne-Claire Duchez,
Nathalie Cloutier,
Tania Levesque,
Frederic Jacques,
Jean Perron,
Peter A. Nigrovic,
Melanie Dieude,
Marie-Josee Hebert

Published: January 14, 2015
https://doi.org/10.1371/journal.pone.0116812

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