Six Amino Acid Residues in a 1200 Å2 Interface Mediate Binding of Factor VIII to an IgG4κ Inhibitory Antibody

Jasper C. Lin; Ruth A. Ettinger; Jason T. Schuman; Ai-Hong Zhang; Muhammad Wamiq-Adhami; Phuong-Cac T. Nguyen; Shelley M. Nakaya-Fletcher; Komal Puranik; Arthur R. Thompson; Kathleen P. Pratt

doi:10.1371/journal.pone.0116577

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Peer-reviewed

Research Article

Six Amino Acid Residues in a 1200 Å2 Interface Mediate Binding of Factor VIII to an IgG4κ Inhibitory Antibody

Jasper C. Lin ,

Current address: Genentech, South San Francisco, California, United States of America

¶‡ These authors contributed equally to this work.

Affiliations Puget Sound Blood Center Research Institute, Seattle, Washington, United States of America, Division of Hematology, Dept. Medicine, University of Washington, Seattle, Washington, United States of America
⨯
Ruth A. Ettinger ,

¶‡ These authors contributed equally to this work.

Affiliation Puget Sound Blood Center Research Institute, Seattle, Washington, United States of America
⨯
Jason T. Schuman,

Affiliation General Electric (GE) Healthcare Life Sciences, Portland, Oregon, United States of America
⨯
Ai-Hong Zhang,

Affiliation Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America
⨯
Muhammad Wamiq-Adhami,

Affiliation Department of Pathology, Walter Reed National Military Medical Center, Bethesda, Maryland, United States of America
⨯
Phuong-Cac T. Nguyen,

Affiliation Puget Sound Blood Center Research Institute, Seattle, Washington, United States of America
⨯
Shelley M. Nakaya-Fletcher,

Affiliation Puget Sound Blood Center Research Institute, Seattle, Washington, United States of America
⨯
Komal Puranik,

Affiliation Puget Sound Blood Center Research Institute, Seattle, Washington, United States of America
⨯
Arthur R. Thompson,

Affiliations Puget Sound Blood Center Research Institute, Seattle, Washington, United States of America, Division of Hematology, Dept. Medicine, University of Washington, Seattle, Washington, United States of America
⨯
Kathleen P. Pratt

* E-mail: Kathleen.pratt@usuhs.edu

Affiliations Puget Sound Blood Center Research Institute, Seattle, Washington, United States of America, Division of Hematology, Dept. Medicine, University of Washington, Seattle, Washington, United States of America, Uniformed Services University of the Health Sciences, Bethesda, Maryland, United States of America
⨯

Six Amino Acid Residues in a 1200 Å2 Interface Mediate Binding of Factor VIII to an IgG4κ Inhibitory Antibody

Jasper C. Lin,
Ruth A. Ettinger,
Jason T. Schuman,
Ai-Hong Zhang,
Muhammad Wamiq-Adhami,
Phuong-Cac T. Nguyen,
Shelley M. Nakaya-Fletcher,
Komal Puranik,
Arthur R. Thompson,
Kathleen P. Pratt

Published: January 23, 2015
https://doi.org/10.1371/journal.pone.0116577

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