Histidine 352 (His352) and Tryptophan 355 (Trp355) Are Essential for Flax UGT74S1 Glucosylation Activity toward Secoisolariciresinol

Open Access

Peer-reviewed

Research Article

Histidine 352 (His³⁵²) and Tryptophan 355 (Trp³⁵⁵) Are Essential for Flax UGT74S1 Glucosylation Activity toward Secoisolariciresinol

Kaushik Ghose,

Affiliations Crops and Livestock Research Centre, Agriculture and Agri-Food Canada, 440 University Avenue, Charlottetown, Prince Edward Island, C1A 4N6, Canada, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island, C1A 4P3, Canada
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Jason McCallum,

Affiliations Crops and Livestock Research Centre, Agriculture and Agri-Food Canada, 440 University Avenue, Charlottetown, Prince Edward Island, C1A 4N6, Canada, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island, C1A 4P3, Canada
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Marva Sweeney-Nixon,

Affiliation University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island, C1A 4P3, Canada
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Bourlaye Fofana

* E-mail: bourlaye.fofana@agr.gc.ca

Affiliations Crops and Livestock Research Centre, Agriculture and Agri-Food Canada, 440 University Avenue, Charlottetown, Prince Edward Island, C1A 4N6, Canada, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island, C1A 4P3, Canada
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Hydrogen bonding
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Oxygen
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Protein structure
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Site-directed mutagenesis
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Flax
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Protein structure prediction
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Protein structure databases
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Mutagenesis
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