Transcription Activator-Like Effector Nuclease (TALEN)-Mediated CLYBL Targeting Enables Enhanced Transgene Expression and One-Step Generation of Dual Reporter Human Induced Pluripotent Stem Cell (iPSC) and Neural Stem Cell (NSC) Lines

Trevor Cerbini; Ray Funahashi; Yongquan Luo; Chengyu Liu; Kyeyoon Park; Mahendra Rao; Nasir Malik; Jizhong Zou

doi:10.1371/journal.pone.0116032

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Transcription Activator-Like Effector Nuclease (TALEN)-Mediated CLYBL Targeting Enables Enhanced Transgene Expression and One-Step Generation of Dual Reporter Human Induced Pluripotent Stem Cell (iPSC) and Neural Stem Cell (NSC) Lines

Trevor Cerbini,

Affiliation NIH Center for Regenerative Medicine, Laboratory of Stem Cell Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, Maryland, United States of America
⨯
Ray Funahashi,

Affiliation NIH Center for Regenerative Medicine, Laboratory of Stem Cell Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, Maryland, United States of America
⨯
Yongquan Luo,

Affiliation NIH Center for Regenerative Medicine, Laboratory of Stem Cell Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, Maryland, United States of America
⨯
Chengyu Liu,

Affiliation Center for Molecular Medicine, Division of Intramural Research, National Heart, Lung, and Blood Institute, Bethesda, Maryland, United States of America
⨯
Kyeyoon Park,

Affiliation Stem Cell Unit, National Institute of Neurological Disorders and Stroke, Bethesda, Maryland, United States of America
⨯
Mahendra Rao,

Affiliation NIH Center for Regenerative Medicine, Laboratory of Stem Cell Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, Maryland, United States of America
⨯
Nasir Malik,

Affiliation NIH Center for Regenerative Medicine, Laboratory of Stem Cell Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, Maryland, United States of America
⨯
Jizhong Zou

* E-mail: zouj2@mail.nih.gov

Affiliations NIH Center for Regenerative Medicine, Laboratory of Stem Cell Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, Maryland, United States of America, Center for Molecular Medicine, Division of Intramural Research, National Heart, Lung, and Blood Institute, Bethesda, Maryland, United States of America
⨯

Transcription Activator-Like Effector Nuclease (TALEN)-Mediated CLYBL Targeting Enables Enhanced Transgene Expression and One-Step Generation of Dual Reporter Human Induced Pluripotent Stem Cell (iPSC) and Neural Stem Cell (NSC) Lines

Trevor Cerbini,
Ray Funahashi,
Yongquan Luo,
Chengyu Liu,
Kyeyoon Park,
Mahendra Rao,
Nasir Malik,
Jizhong Zou

Published: January 14, 2015
https://doi.org/10.1371/journal.pone.0116032

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Cloning
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TALENs
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