Universal Stem-Loop Primer Method for Screening and Quantification of MicroRNA

Li-hong Yang; Si-lu Wang; Li-li Tang; Biao Liu; Wen-le Ye; Ling-ling Wang; Zhang-yang Wang; Meng-tao Zhou; Bi-cheng Chen

doi:10.1371/journal.pone.0115293

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Open Access

Peer-reviewed

Research Article

Universal Stem-Loop Primer Method for Screening and Quantification of MicroRNA

Li-hong Yang ,

¶These authors are co-first authors on this work.

Affiliation Clinical Laboratory Diagnostic Centre, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, Peoples Republic of China
⨯
Si-lu Wang ,

¶These authors are co-first authors on this work.

Affiliation Wenzhou Key Laboratory of Surgery, Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, Peoples Republic of China
⨯
Li-li Tang,

Affiliation Wenzhou Key Laboratory of Surgery, Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, Peoples Republic of China
⨯
Biao Liu,

Affiliation Wenzhou Key Laboratory of Surgery, Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, Peoples Republic of China
⨯
Wen-le Ye,

Affiliation Wenzhou Key Laboratory of Surgery, Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, Peoples Republic of China
⨯
Ling-ling Wang,

Affiliation Clinical Laboratory Diagnostic Centre, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, Peoples Republic of China
⨯
Zhang-yang Wang,

Affiliation Wenzhou Key Laboratory of Surgery, Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, Peoples Republic of China
⨯
Meng-tao Zhou ,

* E-mail: bisonch@163.com

Affiliation Wenzhou Key Laboratory of Surgery, Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, Peoples Republic of China
⨯
Bi-cheng Chen

Affiliation Wenzhou Key Laboratory of Surgery, Department of Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, Peoples Republic of China
⨯

Universal Stem-Loop Primer Method for Screening and Quantification of MicroRNA

Li-hong Yang,
Si-lu Wang,
Li-li Tang,
Biao Liu,
Wen-le Ye,
Ling-ling Wang,
Zhang-yang Wang,
Meng-tao Zhou,
Bi-cheng Chen

Published: December 30, 2014
https://doi.org/10.1371/journal.pone.0115293

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Polymerase chain reaction
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T cells
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MicroRNAs
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Reverse transcription
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Nucleotides
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Gel electrophoresis
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Gene regulation
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Nucleotide sequencing
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