REAL-Select: Full-Length Antibody Display and Library Screening by Surface Capture on Yeast Cells

Open Access

Peer-reviewed

Research Article

Laura Rhiel ,

Contributed equally to this work with: Laura Rhiel, Simon Krah

Affiliation Protein Engineering and Antibody Technologies, Merck Serono, Merck KGaA, Darmstadt, Germany
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Simon Krah ,

Contributed equally to this work with: Laura Rhiel, Simon Krah

Affiliation Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Darmstadt, Germany
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Ralf Günther,

Affiliation Protein Engineering and Antibody Technologies, Merck Serono, Merck KGaA, Darmstadt, Germany
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Stefan Becker,

Affiliation Protein Engineering and Antibody Technologies, Merck Serono, Merck KGaA, Darmstadt, Germany
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Harald Kolmar ,

* E-mail: Bjoern.Hock@merckgroup.com (BH); Kolmar@Biochemie-TUD.de (HK)

Affiliation Institute for Organic Chemistry and Biochemistry, Technische Universität Darmstadt, Darmstadt, Germany
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Björn Hock

* E-mail: Bjoern.Hock@merckgroup.com (BH); Kolmar@Biochemie-TUD.de (HK)

Affiliation Protein Engineering and Antibody Technologies, Merck Serono, Merck KGaA, Darmstadt, Germany
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Yeast
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Saccharomyces cerevisiae
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Library screening
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Flow cytometry
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Cloning
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Cell fusion
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Cell staining
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Cell binding
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