A Simplified and Versatile System for the Simultaneous Expression of Multiple siRNAs in Mammalian Cells Using Gibson DNA Assembly

Fang Deng; Xiang Chen; Zhan Liao; Zhengjian Yan; Zhongliang Wang; Youlin Deng; Qian Zhang; Zhonglin Zhang; Jixing Ye; Min Qiao; Ruifang Li; Sahitya Denduluri; Jing Wang; Qiang Wei; Melissa Li; Nisha Geng; Lianggong Zhao; Guolin Zhou; Penghui Zhang; Hue H. Luu; Rex C. Haydon; Russell R. Reid; Tian Yang; Tong-Chuan He

doi:10.1371/journal.pone.0113064

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Peer-reviewed

Research Article

A Simplified and Versatile System for the Simultaneous Expression of Multiple siRNAs in Mammalian Cells Using Gibson DNA Assembly

Fang Deng,

Affiliations Department of Cell Biology, Third Military Medical University, Chongqing, 400038, China, Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America
⨯
Xiang Chen,

Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America
⨯
Zhan Liao,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Department of Orthopaedic Surgery, the Affiliated Xiang-Ya Hospital of Central South University, Changsha, 410008, China
⨯
Zhengjian Yan,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, 400016, China
⨯
Zhongliang Wang,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, 400016, China
⨯
Youlin Deng,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, 400016, China
⨯
Qian Zhang,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, 400016, China
⨯
Zhonglin Zhang,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Department of Surgery, the Affiliated Zhongnan Hospital of Wuhan University, Wuhan, 430071, China
⨯
Jixing Ye,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, School of Bioengineering, Chongqing University, Chongqing, 400044, China
⨯
Min Qiao,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, 400016, China, Department of Orthopaedic Surgery, the Affiliated Xiang-Ya Hospital of Central South University, Changsha, 410008, China
⨯
Ruifang Li,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Department of Surgery, the Affiliated Zhongnan Hospital of Wuhan University, Wuhan, 430071, China
⨯
Sahitya Denduluri,

Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America
⨯
Jing Wang,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, 400016, China
⨯
Qiang Wei,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, 400016, China
⨯
Melissa Li,

Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America
⨯
Nisha Geng,

Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America
⨯
Lianggong Zhao,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Department of Orthopaedic Surgery, the Second Affiliated Hospital of Lanzhou University, Lanzhou, Gansu, 730000, China
⨯
Guolin Zhou,

Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America
⨯
Penghui Zhang,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, 400016, China
⨯
Hue H. Luu,

Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America
⨯
Rex C. Haydon,

Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America
⨯
Russell R. Reid,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, The Laboratory of Craniofacial Biology, Department of Surgery, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America
⨯
Tian Yang ,

* E-mail: tche@uchicago.edu (TCH); tiany@163.net (TY)

Affiliation Department of Cell Biology, Third Military Medical University, Chongqing, 400038, China
⨯
[ ... ],
Tong-Chuan He

* E-mail: tche@uchicago.edu (TCH); tiany@163.net (TY)

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL, 60637, United States of America, Department of Orthopaedic Surgery, the Affiliated Xiang-Ya Hospital of Central South University, Changsha, 410008, China
⨯
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A Simplified and Versatile System for the Simultaneous Expression of Multiple siRNAs in Mammalian Cells Using Gibson DNA Assembly

Fang Deng,
Xiang Chen,
Zhan Liao,
Zhengjian Yan,
Zhongliang Wang,
Youlin Deng,
Qian Zhang,
Zhonglin Zhang,
Jixing Ye,
Min Qiao

Published: November 14, 2014
https://doi.org/10.1371/journal.pone.0113064

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