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Open Access

Peer-reviewed

Research Article

Protein Thermal Stability Enhancement by Designing Salt Bridges: A Combined Computational and Experimental Study

Chi-Wen Lee ,

Contributed equally to this work with: Chi-Wen Lee, Hsiu-Jung Wang

¶These authors are first authors on this work.

Affiliation Institute of Bioinformatics and Systems Biology, College of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China
⨯
Hsiu-Jung Wang ,

Contributed equally to this work with: Chi-Wen Lee, Hsiu-Jung Wang

¶These authors are first authors on this work.

Affiliation Department of Biological Science and Technology, College of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China
⨯
Jenn-Kang Hwang ,

* E-mail: jkhwang@cc.nctu.edu.tw (JKH); cpts@cc.nctu.edu.tw (CPT)

Affiliation Institute of Bioinformatics and Systems Biology, College of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China
⨯
Ching-Ping Tseng

* E-mail: jkhwang@cc.nctu.edu.tw (JKH); cpts@cc.nctu.edu.tw (CPT)

Affiliation Department of Biological Science and Technology, College of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China
⨯

Protein Thermal Stability Enhancement by Designing Salt Bridges: A Combined Computational and Experimental Study

Chi-Wen Lee,
Hsiu-Jung Wang,
Jenn-Kang Hwang,
Ching-Ping Tseng

x

Published: November 13, 2014
https://doi.org/10.1371/journal.pone.0112751

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Salt bridges
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Thermal stability
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Electrostatics
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Protein structure
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Substitution mutation
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Melting
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Protein interactions
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Packing density
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