The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation

• Janine T. Bossé ,

  * E-mail: j.bosse@imperial.ac.uk

  Affiliation Section of Paediatrics, Imperial College London, St Mary's Campus, London, United Kingdom

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• Denise M. Soares-Bazzolli,

  Affiliations Section of Paediatrics, Imperial College London, St Mary's Campus, London, United Kingdom, Laboratório de Genética Molecular de Micro-organismos, Departamento de Microbiologia - DMB – BIOAGRO, Universidade Federal de Viçosa – Viçosa, Brazil

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• Yanwen Li,

  Affiliation Section of Paediatrics, Imperial College London, St Mary's Campus, London, United Kingdom

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• Brendan W. Wren,

  Affiliation Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, United Kingdom

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• Alexander W. Tucker,

  Affiliation Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

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• Duncan J. Maskell,

  Affiliation Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

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• Andrew N. Rycroft,

  Affiliation Department of Pathology and Pathogen Biology, The Royal Veterinary College, North Mymms, Hatfield, United Kingdom

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• Paul R. Langford,

  Affiliation Section of Paediatrics, Imperial College London, St Mary's Campus, London, United Kingdom

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• on behalf of the BRaDP1T Consortium

  ¶Membership of the BRaDP1T consortium is provided in the Acknowledgments.

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