Rapid Isolation of Extracellular Vesicles from Cell Culture and Biological Fluids Using a Synthetic Peptide with Specific Affinity for Heat Shock Proteins

Anirban Ghosh; Michelle Davey; Ian C. Chute; Steven G. Griffiths; Scott Lewis; Simi Chacko; David Barnett; Nicolas Crapoulet; Sébastien Fournier; Andrew Joy; Michelle C. Caissie; Amanda D. Ferguson; Melissa Daigle; M. Vicki Meli; Stephen M. Lewis; Rodney J. Ouellette

doi:10.1371/journal.pone.0110443

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Peer-reviewed

Research Article

Rapid Isolation of Extracellular Vesicles from Cell Culture and Biological Fluids Using a Synthetic Peptide with Specific Affinity for Heat Shock Proteins

Anirban Ghosh ,

* E-mail: anirbang@canceratl.ca (AG); rodneyo@canceratl.ca (RJO)

Affiliations Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada, Department of Chemistry and Biochemistry, Université de Moncton, Moncton, New Brunswick, Canada
⨯
Michelle Davey,

Affiliation Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada
⨯
Ian C. Chute,

Affiliation Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada
⨯
Steven G. Griffiths,

Affiliation Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada
⨯
Scott Lewis,

Affiliation New England Peptide Inc., Gardner, Massachusetts, United States of America
⨯
Simi Chacko,

Affiliation Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada
⨯
David Barnett,

Affiliations Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada, Department of Chemistry and Biochemistry, Université de Moncton, Moncton, New Brunswick, Canada, Department of Chemistry and Biochemistry, Mount Allison University, Sackville, New Brunswick, Canada
⨯
Nicolas Crapoulet,

Affiliation Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada
⨯
Sébastien Fournier,

Affiliation Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada
⨯
Andrew Joy,

Affiliation Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada
⨯
Michelle C. Caissie,

Affiliation Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada
⨯
Amanda D. Ferguson,

Affiliation Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada
⨯
Melissa Daigle,

Affiliation Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada
⨯
M. Vicki Meli,

Affiliation Department of Chemistry and Biochemistry, Mount Allison University, Sackville, New Brunswick, Canada
⨯
Stephen M. Lewis,

Affiliations Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada, Department of Chemistry and Biochemistry, Université de Moncton, Moncton, New Brunswick, Canada, Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada, Department of Biology, University of New Brunswick, Saint John, New Brunswick, Canada
⨯
[ ... ],
Rodney J. Ouellette

* E-mail: anirbang@canceratl.ca (AG); rodneyo@canceratl.ca (RJO)

Affiliations Atlantic Cancer Research Institute, Moncton, New Brunswick, Canada, Department of Chemistry and Biochemistry, Université de Moncton, Moncton, New Brunswick, Canada
⨯
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Rapid Isolation of Extracellular Vesicles from Cell Culture and Biological Fluids Using a Synthetic Peptide with Specific Affinity for Heat Shock Proteins

Anirban Ghosh,
Michelle Davey,
Ian C. Chute,
Steven G. Griffiths,
Scott Lewis,
Simi Chacko,
David Barnett,
Nicolas Crapoulet,
Sébastien Fournier,
Andrew Joy

Published: October 17, 2014
https://doi.org/10.1371/journal.pone.0110443

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