Improved Genome Editing in Human Cell Lines Using the CRISPR Method

Open Access

Peer-reviewed

Research Article

Ivan M. Munoz,

Affiliation Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, Scotland
⨯
Piotr Szyniarowski,

Affiliation Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, Scotland
⨯
Rachel Toth,

Affiliation Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, Scotland
⨯
John Rouse ,

* E-mail: j.rouse@dundee.ac.uk (JR); c.lachaud@dundee.ac.uk (CL)

Affiliation Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, Scotland
⨯
Christophe Lachaud

* E-mail: j.rouse@dundee.ac.uk (JR); c.lachaud@dundee.ac.uk (CL)

Affiliation Medical Research Council Protein Phosphorylation Unit, College of Life Sciences, University of Dundee, Dundee, Scotland
⨯

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Plasmid construction
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Cloning
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Polymerase chain reaction
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Transfection
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Gene disruption
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DNA repair
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Mutagenesis
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Mutation
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