Development of a Double Antibody Sandwich ELISA for West Nile Virus Detection Using Monoclonal Antibodies against Non-Structural Protein 1

Xi-Xia Ding; Xiao-Feng Li; Yong-Qiang Deng; Yong-Hui Guo; Wei Hao; Xiao-Yan Che; Cheng-Feng Qin; Ning Fu

doi:10.1371/journal.pone.0108623

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Open Access

Peer-reviewed

Research Article

Development of a Double Antibody Sandwich ELISA for West Nile Virus Detection Using Monoclonal Antibodies against Non-Structural Protein 1

Xi-Xia Ding,

Affiliation Laboratory of Emerging Infectious Diseases and Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China
⨯
Xiao-Feng Li,

Affiliation Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
⨯
Yong-Qiang Deng,

Affiliation Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
⨯
Yong-Hui Guo,

Affiliation Laboratory of Emerging Infectious Diseases and Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China
⨯
Wei Hao,

Affiliation Laboratory of Emerging Infectious Diseases and Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China
⨯
Xiao-Yan Che †,

† Deceased.

Affiliation Laboratory of Emerging Infectious Diseases and Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China
⨯
Cheng-Feng Qin ,

* E-mail: chengfeng_qin@126.com (CFQ); zfzn2013@126.com (NF)

Affiliation Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
⨯
Ning Fu

* E-mail: chengfeng_qin@126.com (CFQ); zfzn2013@126.com (NF)

Affiliation Laboratory of Emerging Infectious Diseases and Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China
⨯

Development of a Double Antibody Sandwich ELISA for West Nile Virus Detection Using Monoclonal Antibodies against Non-Structural Protein 1

Xi-Xia Ding,
Xiao-Feng Li,
Yong-Qiang Deng,
Yong-Hui Guo,
Wei Hao,
Xiao-Yan Che,
Cheng-Feng Qin,
Ning Fu

Published: October 10, 2014
https://doi.org/10.1371/journal.pone.0108623

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West Nile virus
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Enzyme-linked immunoassays
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Reverse transcriptase-polymerase chain reaction
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Antibody isotype determination
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