Label-Free and Sensitive Fluorescent Detection of Sequence-Specific Single-Strand DNA Based on S1 Nuclease Cleavage Effects

Zheng Guan; Jinchuan Liu; Wenhui Bai; Zhenzhen Lv; Xiaoling Jiang; Shuming Yang; Ailiang Chen; Guiyuan Lv

doi:10.1371/journal.pone.0108401

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Open Access

Peer-reviewed

Research Article

Label-Free and Sensitive Fluorescent Detection of Sequence-Specific Single-Strand DNA Based on S1 Nuclease Cleavage Effects

Zheng Guan,

Affiliations Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-food Quality and Safety, Ministry of Agriculture, Beijing, China, Institute of Materia Medica, Zhejiang Chinese Medical University, Hangzhou, China
⨯
Jinchuan Liu,

Affiliation Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-food Quality and Safety, Ministry of Agriculture, Beijing, China
⨯
Wenhui Bai,

Affiliation Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-food Quality and Safety, Ministry of Agriculture, Beijing, China
⨯
Zhenzhen Lv,

Affiliation Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-food Quality and Safety, Ministry of Agriculture, Beijing, China
⨯
Xiaoling Jiang,

Affiliation Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-food Quality and Safety, Ministry of Agriculture, Beijing, China
⨯
Shuming Yang,

Affiliation Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-food Quality and Safety, Ministry of Agriculture, Beijing, China
⨯
Ailiang Chen ,

* E-mail: ailiang.chen@gmail.com (AC); guiyuan_lv@yeah.net (GL)

Affiliation Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-food Quality and Safety, Ministry of Agriculture, Beijing, China
⨯
Guiyuan Lv

* E-mail: ailiang.chen@gmail.com (AC); guiyuan_lv@yeah.net (GL)

Affiliation Institute of Materia Medica, Zhejiang Chinese Medical University, Hangzhou, China
⨯

Label-Free and Sensitive Fluorescent Detection of Sequence-Specific Single-Strand DNA Based on S1 Nuclease Cleavage Effects

Zheng Guan,
Jinchuan Liu,
Wenhui Bai,
Zhenzhen Lv,
Xiaoling Jiang,
Shuming Yang,
Ailiang Chen,
Guiyuan Lv

Published: October 6, 2014
https://doi.org/10.1371/journal.pone.0108401

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15 Nov 2014
10:18 GMT
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Some tips to improve your experience by using this method----From: Z. Guan Posted by zhengguancn on 15 Nov 2014 at 10:18 GMT

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