Probing the Crucial Role of Leu31 and Thr33 of the Bacillus pumilus CBS Alkaline Protease in Substrate Recognition and Enzymatic Depilation of Animal Hide

Nadia Zaraî Jaouadi; Bassem Jaouadi; Hajer Ben Hlima; Hatem Rekik; Mouna Belhoul; Maher Hmidi; Houda Slimene Ben Aicha; Chiraz Gorgi Hila; Abdessatar Toumi; Nushin Aghajari; Samir Bejar

doi:10.1371/journal.pone.0108367

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Peer-reviewed

Research Article

Probing the Crucial Role of Leu31 and Thr33 of the Bacillus pumilus CBS Alkaline Protease in Substrate Recognition and Enzymatic Depilation of Animal Hide

Nadia Zaraî Jaouadi,

Affiliation Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia
⨯
Bassem Jaouadi ,

* E-mail: bassem.jaouadi@cbs.rnrt.tn (BJ); samir.bejar@cbs.rnrt.tn (SB)

Affiliation Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia
⨯
Hajer Ben Hlima,

Affiliation Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia
⨯
Hatem Rekik,

Affiliation Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia
⨯
Mouna Belhoul,

Affiliation Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia
⨯
Maher Hmidi,

Affiliation Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia
⨯
Houda Slimene Ben Aicha,

Affiliation National Leather and Shoe Center (CNCC), Mégrine, Ben Arous, Tunisia
⨯
Chiraz Gorgi Hila,

Affiliation National Leather and Shoe Center (CNCC), Mégrine, Ben Arous, Tunisia
⨯
Abdessatar Toumi,

Affiliation National Leather and Shoe Center (CNCC), Mégrine, Ben Arous, Tunisia
⨯
Nushin Aghajari,

Affiliation Laboratory for Biocrystallography and Structural Biology of Therapeutic Targets, Molecular and Structural Bases of Infectious Systems, UMR 5086-CNRS-University of Lyon 1, Institute for the Biology and Chemistry of Proteins (IBCP), FR3302, Lyon, France
⨯
Samir Bejar

* E-mail: bassem.jaouadi@cbs.rnrt.tn (BJ); samir.bejar@cbs.rnrt.tn (SB)

Affiliation Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax (CBS), University of Sfax, Sfax, Tunisia
⨯

Probing the Crucial Role of Leu31 and Thr33 of the Bacillus pumilus CBS Alkaline Protease in Substrate Recognition and Enzymatic Depilation of Animal Hide

Nadia Zaraî Jaouadi,
Bassem Jaouadi,
Hajer Ben Hlima,
Hatem Rekik,
Mouna Belhoul,
Maher Hmidi,
Houda Slimene Ben Aicha,
Chiraz Gorgi Hila,
Abdessatar Toumi,
Nushin Aghajari

Published: September 29, 2014
https://doi.org/10.1371/journal.pone.0108367

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