The Meganuclease I-SceI Containing Nuclear Localization Signal (NLS-I-SceI) Efficiently Mediated Mammalian Germline Transgenesis via Embryo Cytoplasmic Microinjection

Open Access

Peer-reviewed

Research Article

Yong Wang ,

Contributed equally to this work with: Yong Wang, Xiao-Yang Zhou

* E-mail: yongw7528@gmail.com (YW); weihong63528@163.com (HW)

Affiliation Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China
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Xiao-Yang Zhou ,

Contributed equally to this work with: Yong Wang, Xiao-Yang Zhou

Affiliation Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China
⨯
Peng-Ying Xiang,

Affiliation Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China
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Lu-Lu Wang,

Affiliation Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China
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Huan Tang,

Affiliation China Three Gorges Museum, Chongqing, China
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Fei Xie,

Affiliation Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China
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Liang Li,

Affiliation Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China
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Hong Wei

* E-mail: yongw7528@gmail.com (YW); weihong63528@163.com (HW)

Affiliation Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing, China
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Embryos
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Genetically modified animals
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Microinjection
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Plasmid construction
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Swine
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Eggs
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Mammalian genomics
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Cytoplasm
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