Combining Rational and Random Strategies in β-Glucosidase Zm-p60.1 Protein Library Construction

Open Access

Peer-reviewed

Research Article

Dušan Turek,

Affiliation Laboratory of Plant Molecular Biology, Institute of Biophysics AS CR, v.v.i. CEITEC – Central European Institute of Technology, Mendel University in Brno, Brno, Czech Republic
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Pavel Klimeš,

Affiliation Laboratory of Plant Molecular Biology, Institute of Biophysics AS CR, v.v.i. CEITEC – Central European Institute of Technology, Mendel University in Brno, Brno, Czech Republic
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Pavel Mazura ,

* E-mail: mazura@sci.muni.cz

Affiliation Laboratory of Plant Molecular Biology, Institute of Biophysics AS CR, v.v.i. CEITEC – Central European Institute of Technology, Mendel University in Brno, Brno, Czech Republic
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Břetislav Brzobohatý

Affiliation Laboratory of Plant Molecular Biology, Institute of Biophysics AS CR, v.v.i. CEITEC – Central European Institute of Technology, Mendel University in Brno, Brno, Czech Republic
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Mutagenesis
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Cloning
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Library screening
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Amino acid analysis
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Site-directed mutagenesis
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Monte Carlo method
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Sequence alignment
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Enzymes
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