In Vivo Mutational Characterization of DndE Involved in DNA Phosphorothioate Modification

Chongde Lai; Xiaolin Wu; Chao Chen; Teng Huang; Xiaolin Xiong; Shuangju Wu; Meijia Gu; Zixin Deng; Xi Chen; Shi Chen; Lianrong Wang

doi:10.1371/journal.pone.0107981

Loading metrics

Open Access

Peer-reviewed

Research Article

In Vivo Mutational Characterization of DndE Involved in DNA Phosphorothioate Modification

Chongde Lai,

Affiliations Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China
⨯
Xiaolin Wu,

Affiliations Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China
⨯
Chao Chen,

Affiliation Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China
⨯
Teng Huang,

Affiliation Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China
⨯
Xiaolin Xiong,

Affiliation Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China
⨯
Shuangju Wu,

Affiliation Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China
⨯
Meijia Gu,

Affiliation Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China
⨯
Zixin Deng,

Affiliation Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China
⨯
Xi Chen,

Affiliation Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China
⨯
Shi Chen ,

* E-mail: shichen@whu.edu.cn (SC); lianrong@whu.edu.cn (LW)

Affiliation Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China
⨯
Lianrong Wang

* E-mail: shichen@whu.edu.cn (SC); lianrong@whu.edu.cn (LW)

Affiliation Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan, China
⨯

In Vivo Mutational Characterization of DndE Involved in DNA Phosphorothioate Modification

Chongde Lai,
Xiaolin Wu,
Chao Chen,
Teng Huang,
Xiaolin Xiong,
Shuangju Wu,
Meijia Gu,
Zixin Deng,
Xi Chen,
Shi Chen

Published: September 30, 2014
https://doi.org/10.1371/journal.pone.0107981

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

DNA modification
Is the Subject Area "DNA modification" applicable to this article?

Thanks for your feedback.
Lysine
Is the Subject Area "Lysine" applicable to this article?

Thanks for your feedback.
Salmonella enterica
Is the Subject Area "Salmonella enterica" applicable to this article?

Thanks for your feedback.
DNA-binding proteins
Is the Subject Area "DNA-binding proteins" applicable to this article?

Thanks for your feedback.
Plasmid construction
Is the Subject Area "Plasmid construction" applicable to this article?

Thanks for your feedback.
Mutation
Is the Subject Area "Mutation" applicable to this article?

Thanks for your feedback.
Alanine
Is the Subject Area "Alanine" applicable to this article?

Thanks for your feedback.
Site-directed mutagenesis
Is the Subject Area "Site-directed mutagenesis" applicable to this article?

Thanks for your feedback.