TaqMan Real-Time PCR Assays for Single-Nucleotide Polymorphisms Which Identify Francisella tularensis and Its Subspecies and Subpopulations

Dawn N. Birdsell; Amy J. Vogler; Jordan Buchhagen; Ashley Clare; Emily Kaufman; Amber Naumann; Elizabeth Driebe; David M. Wagner; Paul S. Keim

doi:10.1371/journal.pone.0107964

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Peer-reviewed

Research Article

TaqMan Real-Time PCR Assays for Single-Nucleotide Polymorphisms Which Identify Francisella tularensis and Its Subspecies and Subpopulations

Dawn N. Birdsell,

Affiliation Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America
⨯
Amy J. Vogler,

Affiliation Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America
⨯
Jordan Buchhagen,

Affiliations Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America, Translational Genomics Research Institute, Flagstaff, Arizona, United States of America
⨯
Ashley Clare,

Affiliation Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America
⨯
Emily Kaufman,

Affiliation Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America
⨯
Amber Naumann,

Affiliation Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America
⨯
Elizabeth Driebe,

Affiliations Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America, Translational Genomics Research Institute, Flagstaff, Arizona, United States of America
⨯
David M. Wagner,

Affiliation Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America
⨯
Paul S. Keim

* E-mail: Paul.Keim@nau.edu

Affiliations Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, Arizona, United States of America, Translational Genomics Research Institute, Flagstaff, Arizona, United States of America
⨯

TaqMan Real-Time PCR Assays for Single-Nucleotide Polymorphisms Which Identify Francisella tularensis and Its Subspecies and Subpopulations

Dawn N. Birdsell,
Amy J. Vogler,
Jordan Buchhagen,
Ashley Clare,
Emily Kaufman,
Amber Naumann,
Elizabeth Driebe,
David M. Wagner,
Paul S. Keim

Published: September 19, 2014
https://doi.org/10.1371/journal.pone.0107964

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Francisella tularensis
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Genetics
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Polymerase chain reaction
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Single nucleotide polymorphisms
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Phylogeography
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DNA
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Genotyping
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Whole genome amplification
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