IRDL Cloning: A One-Tube, Zero-Background, Easy-to-Use, Directional Cloning Method Improves Throughput in Recombinant DNA Preparation

Open Access

Peer-reviewed

Research Article

Jiancai Wang,

Affiliations School of Life Sciences, University of Science and Technology of China, Hefei, People's Republic of China, Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, People's Republic of China
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Ronghua Xu ,

* E-mail: ronghua.xu08@gmail.com (RX); aizhongliu@xtbg.ac.cn (AL)

Affiliation Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, People's Republic of China
⨯
Aizhong Liu

* E-mail: ronghua.xu08@gmail.com (RX); aizhongliu@xtbg.ac.cn (AL)

Affiliations Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, People's Republic of China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Kunming, People's Republic of China
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Cloning
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Polymerase chain reaction
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Vector cloning
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DNA cloning
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Gene cloning
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Plasmid construction
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Vector construction
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Directional cloning
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