Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

Open Access

Peer-reviewed

Research Article

Melanie Spornraft ,

* E-mail: melanie.spornraft@wzw.tum.de

Current address: Physiology Weihenstephan and ZIEL Research Center for Nutrition and Food Sciences, Technical University Munich, Freising, Germany

Affiliation Physiology Weihenstephan and ZIEL Research Center for Nutrition and Food Sciences, Technical University Munich, Freising, Germany
⨯
Benedikt Kirchner,

Affiliation Physiology Weihenstephan and ZIEL Research Center for Nutrition and Food Sciences, Technical University Munich, Freising, Germany
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Bettina Haase,

Affiliation Genomics Core Facility, EMBL European Molecular Biology Laboratory, Heidelberg, Germany
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Vladimir Benes,

Affiliation Genomics Core Facility, EMBL European Molecular Biology Laboratory, Heidelberg, Germany
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Michael W. Pfaffl,

Affiliation Physiology Weihenstephan and ZIEL Research Center for Nutrition and Food Sciences, Technical University Munich, Freising, Germany
⨯
Irmgard Riedmaier

Affiliation Physiology Weihenstephan and ZIEL Research Center for Nutrition and Food Sciences, Technical University Munich, Freising, Germany
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MicroRNAs
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RNA sequencing
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Blood plasma
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RNA extraction
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Biomarkers
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Small nucleolar RNA
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RNA analysis
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DNA libraries
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