Usefulness of a Darwinian System in a Biotechnological Application: Evolution of Optical Window Fluorescent Protein Variants under Selective Pressure

• Ulrike Schoetz ,

  Contributed equally to this work with: Ulrike Schoetz, Nikolaos C. Deliolanis

  Current address: Department of Radiation Oncology, Ludwig-Maximilians-Universität München, Munich, Germany; Clinical Cooperation Group ‘Personalized Radiotherapy of Head and Neck Cancer’, Helmholtz Center Munich - German Research Center for Environmental Health (GmbH), Munich, Germany

  Affiliation Department of Radiation Sciences - Research Unit Radiation Cytogenetics, Helmholtz Center Munich - German Research Center for Environmental Health (GmbH), Munich, Germany

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• Nikolaos C. Deliolanis ,

  Contributed equally to this work with: Ulrike Schoetz, Nikolaos C. Deliolanis

  Current address: Fraunhofer Project Group for Automation in Medicine and Biotechnology (PAMB), Mannheim, Germany

  Affiliation Institute for Biological and Medical Imaging, Helmholtz Center Munich - German Research Center for Environmental Health (GmbH), Munich, Germany

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• David Ng,

  Affiliation Research Group Cellular Dynamics, Max Planck Institute of Neurobiology, Martinsried, Germany

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• Jutta Pauli,

  Affiliation BAM Federal Institute for Materials Research and Testing, Division Biophotonics, Berlin, Germany

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• Ute Resch-Genger,

  Affiliation BAM Federal Institute for Materials Research and Testing, Division Biophotonics, Berlin, Germany

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• Enrico Kühn,

  Current address: Department of Genes – Circuits – Behavior, Max Planck Institute of Neurobiology, Martinsried, Germany

  Affiliation Institute of Developmental Genetics, Helmholtz Center Munich - German Research Center for Environmental Health (GmbH), Munich, Germany

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• Steffen Heuer,

  Affiliation Department of Radiation Sciences - Research Unit Radiation Cytogenetics, Helmholtz Center Munich - German Research Center for Environmental Health (GmbH), Munich, Germany

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• Wolfgang Beisker,

  Affiliation Institute for Toxicology, Helmholtz Center Munich - German Research Center for Environmental Health (GmbH), Munich, Germany

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• Reinhard W. Köster,

  Current address: Zoological Institute, TU Braunschweig, Braunschweig, Germany

  Affiliation Institute of Developmental Genetics, Helmholtz Center Munich - German Research Center for Environmental Health (GmbH), Munich, Germany

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• Horst Zitzelsberger,

  Affiliation Department of Radiation Sciences - Research Unit Radiation Cytogenetics, Helmholtz Center Munich - German Research Center for Environmental Health (GmbH), Munich, Germany

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• Randolph B Caldwell

  * E-mail: randolph.caldwell@helmholtz-muenchen.de

  Affiliation Department of Radiation Sciences - Research Unit Radiation Cytogenetics, Helmholtz Center Munich - German Research Center for Environmental Health (GmbH), Munich, Germany

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