Development and Evaluation of a Panel of Filovirus Sequence Capture Probes for Pathogen Detection by Next-Generation Sequencing

Jeffrey W. Koehler; Adrienne T. Hall; P. Alexander Rolfe; Anna N. Honko; Gustavo F. Palacios; Joseph N. Fair; Jean-Jacques Muyembe; Prime Mulembekani; Randal J. Schoepp; Adeyemi Adesokan; Timothy D. Minogue

doi:10.1371/journal.pone.0107007

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Open Access

Peer-reviewed

Research Article

Development and Evaluation of a Panel of Filovirus Sequence Capture Probes for Pathogen Detection by Next-Generation Sequencing

Jeffrey W. Koehler,

Affiliation Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, United States of America
⨯
Adrienne T. Hall,

Affiliation Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, United States of America
⨯
P. Alexander Rolfe,

Affiliation Pathogenica, Inc., Boston, Massachusetts, United States of America
⨯
Anna N. Honko,

Affiliation Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, United States of America
⨯
Gustavo F. Palacios,

Affiliation Center for Genomic Sciences, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, United States of America
⨯
Joseph N. Fair,

Affiliation Metabiota, San Francisco, California, United States of America
⨯
Jean-Jacques Muyembe,

Affiliation Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of the Congo
⨯
Prime Mulembekani,

Affiliation Kinshasa School of Public Health, Kinshasa, Democratic Republic of the Congo
⨯
Randal J. Schoepp,

Affiliation Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, United States of America
⨯
Adeyemi Adesokan,

Affiliation Pathogenica, Inc., Boston, Massachusetts, United States of America
⨯
Timothy D. Minogue

* E-mail: timothy.d.minogue.civ@mail.mil

Affiliation Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, United States of America
⨯

Development and Evaluation of a Panel of Filovirus Sequence Capture Probes for Pathogen Detection by Next-Generation Sequencing

Jeffrey W. Koehler,
Adrienne T. Hall,
P. Alexander Rolfe,
Anna N. Honko,
Gustavo F. Palacios,
Joseph N. Fair,
Jean-Jacques Muyembe,
Prime Mulembekani,
Randal J. Schoepp,
Adeyemi Adesokan

Published: September 10, 2014
https://doi.org/10.1371/journal.pone.0107007

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Ebola virus
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Polymerase chain reaction
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Filoviruses
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Cell cultures
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Probe hybridization
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Signal amplification
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Next-generation sequencing
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