3′ IsomiR Species and DNA Contamination Influence Reliable Quantification of MicroRNAs by Stem-Loop Quantitative PCR

Open Access

Peer-reviewed

Research Article

Anita Schamberger,

Affiliation Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary
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Tamás I. Orbán

* E-mail: orban.tamas@ttk.mta.hu

Affiliations Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary, Chemical Technology Transfer Ltd., Budapest, Hungary
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MicroRNAs
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Polymerase chain reaction
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Reverse transcription
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RNA isolation
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HeLa cells
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Deoxyribonucleases
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Transfection
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Small nuclear RNA
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