Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System

Open Access

Peer-reviewed

Research Article

Kai Li,

Affiliation Deparment of Cardiology, Boston Children’s Hospital, Boston, MA, United States of America
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Gang Wang,

Affiliation Deparment of Cardiology, Boston Children’s Hospital, Boston, MA, United States of America
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Troels Andersen,

Affiliation University of Copenhagen, Copenhagen, Denmark
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Pingzhu Zhou,

Affiliation Deparment of Cardiology, Boston Children’s Hospital, Boston, MA, United States of America
⨯
William T. Pu

* E-mail: wpu@pulab.org

Affiliations Deparment of Cardiology, Boston Children’s Hospital, Boston, MA, United States of America, Harvard Stem Cell Institute, Harvard University, Cambridge, MA, United States of America
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Guide RNA
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Transfection
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Cloning
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Polymerase chain reaction
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Genomics
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Genotyping
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CRISPR
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Nucleases
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