Development and Validation of an Haemophilus influenzae Supragenome Hybridization (SGH) Array for Transcriptomic Analyses

Benjamin A. Janto; N. Luisa Hiller; Rory A. Eutsey; Margaret E. Dahlgren; Joshua P. Earl; Evan Powell; Azad Ahmed; Fen Z. Hu; Garth D. Ehrlich

doi:10.1371/journal.pone.0105493

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Open Access

Peer-reviewed

Research Article

Development and Validation of an Haemophilus influenzae Supragenome Hybridization (SGH) Array for Transcriptomic Analyses

Benjamin A. Janto,

Affiliations Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of America, Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania, United States of America
⨯
N. Luisa Hiller,

Affiliations Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of America, Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States of America
⨯
Rory A. Eutsey,

Affiliation Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of America
⨯
Margaret E. Dahlgren,

Affiliation Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of America
⨯
Joshua P. Earl,

Affiliation Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of America
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Evan Powell,

Affiliation Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of America
⨯
Azad Ahmed,

Affiliation Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of America
⨯
Fen Z. Hu ,

* E-mail: garth.ehrlich@drexelmed.edu (GDE); fhu168168@gmail.com (FZH)

Affiliations Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of America, Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania, United States of America, Department of Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania, United States of America
⨯
Garth D. Ehrlich

* E-mail: garth.ehrlich@drexelmed.edu (GDE); fhu168168@gmail.com (FZH)

Affiliations Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of America, Department of Microbiology and Immunology, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania, United States of America, Department of Otolaryngology Head and Neck Surgery, Drexel University College of Medicine, Allegheny Campus, Pittsburgh, Pennsylvania, United States of America
⨯

Development and Validation of an Haemophilus influenzae Supragenome Hybridization (SGH) Array for Transcriptomic Analyses

Benjamin A. Janto,
N. Luisa Hiller,
Rory A. Eutsey,
Margaret E. Dahlgren,
Joshua P. Earl,
Evan Powell,
Azad Ahmed,
Fen Z. Hu,
Garth D. Ehrlich

Published: October 7, 2014
https://doi.org/10.1371/journal.pone.0105493

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Microarrays
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Haemophilus influenzae
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Genomics
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Bacterial genomics
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RNA hybridization
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RNA extraction
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Transcriptome analysis
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