Development of a Nuclear Transformation System for Oleaginous Green Alga Lobosphaera (Parietochloris) incisa and Genetic Complementation of a Mutant Strain, Deficient in Arachidonic Acid Biosynthesis

Boris Zorin; Omer Grundman; Inna Khozin-Goldberg; Stefan Leu; Michal Shapira; Yuval Kaye; Nicolas Tourasse; Olivier Vallon; Sammy Boussiba

doi:10.1371/journal.pone.0105223

Loading metrics

Open Access

Peer-reviewed

Research Article

Development of a Nuclear Transformation System for Oleaginous Green Alga Lobosphaera (Parietochloris) incisa and Genetic Complementation of a Mutant Strain, Deficient in Arachidonic Acid Biosynthesis

Boris Zorin ,

Contributed equally to this work with: Boris Zorin, Omer Grundman

Affiliation Microalgal Biotechnology Laboratory, French Associates Institute for Agriculture and Biotechnology of Drylands, J. Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel
⨯
Omer Grundman ,

Contributed equally to this work with: Boris Zorin, Omer Grundman

Affiliation Microalgal Biotechnology Laboratory, French Associates Institute for Agriculture and Biotechnology of Drylands, J. Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel
⨯
Inna Khozin-Goldberg ,

* E-mail: khozin@bgu.ac.il

Affiliation Microalgal Biotechnology Laboratory, French Associates Institute for Agriculture and Biotechnology of Drylands, J. Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel
⨯
Stefan Leu,

Affiliation Microalgal Biotechnology Laboratory, French Associates Institute for Agriculture and Biotechnology of Drylands, J. Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel
⨯
Michal Shapira,

Affiliation Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel
⨯
Yuval Kaye,

Affiliation Microalgal Biotechnology Laboratory, French Associates Institute for Agriculture and Biotechnology of Drylands, J. Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel
⨯
Nicolas Tourasse,

Affiliation UMR 7141 CNRS/Université Pierre et Marie Curie, Institut de Biologie Physico-Chimique, Paris, France
⨯
Olivier Vallon,

Affiliation UMR 7141 CNRS/Université Pierre et Marie Curie, Institut de Biologie Physico-Chimique, Paris, France
⨯
Sammy Boussiba

Affiliation Microalgal Biotechnology Laboratory, French Associates Institute for Agriculture and Biotechnology of Drylands, J. Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Midreshet Ben-Gurion, Israel
⨯

Development of a Nuclear Transformation System for Oleaginous Green Alga Lobosphaera (Parietochloris) incisa and Genetic Complementation of a Mutant Strain, Deficient in Arachidonic Acid Biosynthesis

Boris Zorin,
Omer Grundman,
Inna Khozin-Goldberg,
Stefan Leu,
Michal Shapira,
Yuval Kaye,
Nicolas Tourasse,
Olivier Vallon,
Sammy Boussiba

Published: August 18, 2014
https://doi.org/10.1371/journal.pone.0105223

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Algae
Is the Subject Area "Algae" applicable to this article?

Thanks for your feedback.
Polymerase chain reaction
Is the Subject Area "Polymerase chain reaction" applicable to this article?

Thanks for your feedback.
Chlamydomonas reinhardtii
Is the Subject Area "Chlamydomonas reinhardtii" applicable to this article?

Thanks for your feedback.
Cloning
Is the Subject Area "Cloning" applicable to this article?

Thanks for your feedback.
DNA cloning
Is the Subject Area "DNA cloning" applicable to this article?

Thanks for your feedback.
Selection markers
Is the Subject Area "Selection markers" applicable to this article?

Thanks for your feedback.
Genomics
Is the Subject Area "Genomics" applicable to this article?

Thanks for your feedback.
Biosynthesis
Is the Subject Area "Biosynthesis" applicable to this article?

Thanks for your feedback.