Detection of MPLW515L/K Mutations and Determination of Allele Frequencies with a Single-Tube PCR Assay

Hiraku Takei; Soji Morishita; Marito Araki; Yoko Edahiro; Yoshitaka Sunami; Yumi Hironaka; Naohiro Noda; Yuji Sekiguchi; Satoshi Tsuneda; Akimichi Ohsaka; Norio Komatsu

doi:10.1371/journal.pone.0104958

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Open Access

Peer-reviewed

Research Article

Detection of MPLW515L/K Mutations and Determination of Allele Frequencies with a Single-Tube PCR Assay

Hiraku Takei,

Affiliations Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan, Department of Life Science and Medical Bioscience, Waseda University, Tokyo, Japan
⨯
Soji Morishita,

Affiliation Department of Transfusion Medicine and Stem Cell Regulation, Juntendo University Graduate School of Medicine, Tokyo, Japan
⨯
Marito Araki,

Affiliation Department of Transfusion Medicine and Stem Cell Regulation, Juntendo University Graduate School of Medicine, Tokyo, Japan
⨯
Yoko Edahiro,

Affiliation Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan
⨯
Yoshitaka Sunami,

Affiliation Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan
⨯
Yumi Hironaka,

Affiliation Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan
⨯
Naohiro Noda,

Affiliation Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan
⨯
Yuji Sekiguchi,

Affiliation Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan
⨯
Satoshi Tsuneda,

Affiliation Department of Life Science and Medical Bioscience, Waseda University, Tokyo, Japan
⨯
Akimichi Ohsaka,

Affiliation Department of Transfusion Medicine and Stem Cell Regulation, Juntendo University Graduate School of Medicine, Tokyo, Japan
⨯
Norio Komatsu

* E-mail: komatsun@juntendo.ac.jp

Affiliation Department of Hematology, Juntendo University School of Medicine, Tokyo, Japan
⨯

Detection of MPLW515L/K Mutations and Determination of Allele Frequencies with a Single-Tube PCR Assay

Hiraku Takei,
Soji Morishita,
Marito Araki,
Yoko Edahiro,
Yoshitaka Sunami,
Yumi Hironaka,
Naohiro Noda,
Yuji Sekiguchi,
Satoshi Tsuneda,
Akimichi Ohsaka

Published: August 21, 2014
https://doi.org/10.1371/journal.pone.0104958

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17 Nov 2014
12:09 GMT
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A correction of the article Posted by htakei on 17 Nov 2014 at 12:09 GMT

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Mutation detection
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Amplification-refractory mutation system analysis
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Polymerase chain reaction
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Human genomics
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Signal amplification
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DNA purification
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Substitution mutation
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