Decitabine Suspends Human CD34+ Cell Differentiation and Proliferation during Lentiviral Transduction

Open Access

Peer-reviewed

Research Article

Decitabine Suspends Human CD34⁺ Cell Differentiation and Proliferation during Lentiviral Transduction

Naoya Uchida,

Affiliation Molecular and Clinical Hematology Branch, National Heart Lung and Blood Institutes (NHLBI)/National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, Maryland, United States of America
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Matthew M. Hsieh,

Affiliation Molecular and Clinical Hematology Branch, National Heart Lung and Blood Institutes (NHLBI)/National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, Maryland, United States of America
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Charlotte Platner,

Affiliation Molecular and Clinical Hematology Branch, National Heart Lung and Blood Institutes (NHLBI)/National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, Maryland, United States of America
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Yogen Saunthararajah,

Affiliation Translational Hematology and Oncology Research, Taussig Cancer Institute, Cleveland Clinic, Cleveland, Ohio, United States of America
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John F. Tisdale

* E-mail: johntis@mail.nih.gov

Affiliation Molecular and Clinical Hematology Branch, National Heart Lung and Blood Institutes (NHLBI)/National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, Maryland, United States of America
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Cell differentiation
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Cytokines
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Cell enumeration techniques
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HIV-1
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Hematopoietic stem cells
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Viable cell counting
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Flow cytometry
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Stem cell therapy
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