A High-Throughput Assay for Small Molecule Destabilizers of the KRAS Oncoprotein

Joseph Carver; Thomas S. Dexheimer; Dennis Hsu; Meng-Tzu Weng; Jordan L. Smith; Rajarshi Guha; Ajit Jadhav; Anton Simeonov; Ji Luo

doi:10.1371/journal.pone.0103836

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Open Access

Peer-reviewed

Research Article

A High-Throughput Assay for Small Molecule Destabilizers of the KRAS Oncoprotein

Joseph Carver,

Affiliation Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
Thomas S. Dexheimer,

Affiliation Division of Preclinical Innovation, National Institutes of Health Chemical Genomics Center, National Center for Advancing Translational Sciences, Bethesda, Maryland, United States of America
⨯
Dennis Hsu,

Current address: Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan, United States of America

Affiliation Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
Meng-Tzu Weng,

Current address: Graduate Institute of Clinical Medicine, National Taiwan University and Far-Eastern Memorial Hospital, Taipei, Taiwan

Affiliation Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
Jordan L. Smith,

Affiliation Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America
⨯
Rajarshi Guha,

Affiliation Division of Preclinical Innovation, National Institutes of Health Chemical Genomics Center, National Center for Advancing Translational Sciences, Bethesda, Maryland, United States of America
⨯
Ajit Jadhav,

Affiliation Division of Preclinical Innovation, National Institutes of Health Chemical Genomics Center, National Center for Advancing Translational Sciences, Bethesda, Maryland, United States of America
⨯
Anton Simeonov ,

* E-mail: asimeono@mail.nih.gov (AS); ji.luo@nih.gov (JL)

Affiliation Division of Preclinical Innovation, National Institutes of Health Chemical Genomics Center, National Center for Advancing Translational Sciences, Bethesda, Maryland, United States of America
⨯
Ji Luo

* E-mail: asimeono@mail.nih.gov (AS); ji.luo@nih.gov (JL)

Affiliation Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America
⨯

A High-Throughput Assay for Small Molecule Destabilizers of the KRAS Oncoprotein

Joseph Carver,
Thomas S. Dexheimer,
Dennis Hsu,
Meng-Tzu Weng,
Jordan L. Smith,
Rajarshi Guha,
Ajit Jadhav,
Anton Simeonov,
Ji Luo

Published: August 5, 2014
https://doi.org/10.1371/journal.pone.0103836

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