Expansion of IgG+ B-Cells during Mitogen Stimulation for Memory B-Cell ELISpot Analysis Is Influenced by Size and Composition of the B-Cell Pool

Open Access

Peer-reviewed

Research Article

Anja Scholzen ,

Contributed equally to this work with: Anja Scholzen, Wiebke Nahrendorf

* E-mail: anja.scholzen@radboudumc.nl (AS); robert.sauerwein@radboudumc.nl (RWS)

Affiliation Department of Medical Microbiology, Radboud university medical center, Nijmegen, The Netherlands
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Wiebke Nahrendorf ,

Contributed equally to this work with: Anja Scholzen, Wiebke Nahrendorf

Affiliations Department of Medical Microbiology, Radboud university medical center, Nijmegen, The Netherlands, Division of Parasitology, MRC National Institute for Medical Research, London, United Kingdom
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Jean Langhorne,

Affiliation Division of Parasitology, MRC National Institute for Medical Research, London, United Kingdom
⨯
Robert W. Sauerwein

* E-mail: anja.scholzen@radboudumc.nl (AS); robert.sauerwein@radboudumc.nl (RWS)

Affiliation Department of Medical Microbiology, Radboud university medical center, Nijmegen, The Netherlands
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B cells
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Mitogens
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Enzyme-linked immunoassays
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Flow cytometry
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Cell staining
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Cell differentiation
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Chloroquine
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