Directed Differentiation of Patient-Specific Induced Pluripotent Stem Cells Identifies the Transcriptional Repression and Epigenetic Modification of NKX2-5, HAND1, and NOTCH1 in Hypoplastic Left Heart Syndrome

Junko Kobayashi; Masashi Yoshida; Suguru Tarui; Masataka Hirata; Yusuke Nagai; Shingo Kasahara; Keiji Naruse; Hiroshi Ito; Shunji Sano; Hidemasa Oh

doi:10.1371/journal.pone.0102796

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Research Article

Directed Differentiation of Patient-Specific Induced Pluripotent Stem Cells Identifies the Transcriptional Repression and Epigenetic Modification of NKX2-5, HAND1, and NOTCH1 in Hypoplastic Left Heart Syndrome

Junko Kobayashi,

Affiliation Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
⨯
Masashi Yoshida,

Affiliation Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
⨯
Suguru Tarui,

Affiliation Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
⨯
Masataka Hirata,

Affiliation Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
⨯
Yusuke Nagai,

Affiliation Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
⨯
Shingo Kasahara,

Affiliation Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
⨯
Keiji Naruse,

Affiliation Department of Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
⨯
Hiroshi Ito,

Affiliation Department of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
⨯
Shunji Sano,

Affiliation Department of Cardiovascular Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan
⨯
Hidemasa Oh

* E-mail: hidemasa@md.okayama-u.ac.jp

Affiliation Department of Regenerative Medicine, Center for Innovative Clinical Medicine, Okayama University Hospital, Okayama, Japan
⨯

Directed Differentiation of Patient-Specific Induced Pluripotent Stem Cells Identifies the Transcriptional Repression and Epigenetic Modification of NKX2-5, HAND1, and NOTCH1 in Hypoplastic Left Heart Syndrome

Junko Kobayashi,
Masashi Yoshida,
Suguru Tarui,
Masataka Hirata,
Yusuke Nagai,
Shingo Kasahara,
Keiji Naruse,
Hiroshi Ito,
Shunji Sano,
Hidemasa Oh

Published: July 22, 2014
https://doi.org/10.1371/journal.pone.0102796

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Induced pluripotent stem cells
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Cell differentiation
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DNA transcription
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Heart
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Transcription factors
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Transfection
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Cardiomyocytes
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Gene expression
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