Long-Term Culture of Rat Hippocampal Neurons at Low Density in Serum-Free Medium: Combination of the Sandwich Culture Technique with the Three-Dimensional Nanofibrous Hydrogel PuraMatrix

Open Access

Peer-reviewed

Research Article

Ai Kaneko ,

* E-mail: kaneko@ccr.tsukuba.ac.jp

Affiliation Center for Cybernics Research, University of Tsukuba, Tsukuba, Japan
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Yoshiyuki Sankai

Affiliation Center for Cybernics Research, University of Tsukuba, Tsukuba, Japan
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Neurites
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Neurons
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Fluorescence imaging
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Hippocampus
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Astrocytes
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Axons
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Neuronal differentiation
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Neuronal dendrites
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