Structure-Based Rational Design to Enhance the Solubility and Thermostability of a Bacterial Laccase Lac15

Zemin Fang; Peng Zhou; Fei Chang; Qiang Yin; Wei Fang; Jing Yuan; Xuecheng Zhang; Yazhong Xiao

doi:10.1371/journal.pone.0102423

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Open Access

Peer-reviewed

Research Article

Structure-Based Rational Design to Enhance the Solubility and Thermostability of a Bacterial Laccase Lac15

Zemin Fang,

Affiliations School of Life Sciences, Anhui University, Hefei, Anhui, China, Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui, China
⨯
Peng Zhou,

Affiliations School of Life Sciences, Anhui University, Hefei, Anhui, China, Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui, China
⨯
Fei Chang,

Affiliations School of Life Sciences, Anhui University, Hefei, Anhui, China, Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui, China
⨯
Qiang Yin,

Affiliations School of Life Sciences, Anhui University, Hefei, Anhui, China, Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui, China
⨯
Wei Fang,

Affiliations School of Life Sciences, Anhui University, Hefei, Anhui, China, Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui, China
⨯
Jing Yuan,

Affiliations School of Life Sciences, Anhui University, Hefei, Anhui, China, Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui, China
⨯
Xuecheng Zhang ,

* E-mail: turenzh@ahu.edu.cn (XZ); yzxiao@ahu.edu.cn (YX)

Affiliations School of Life Sciences, Anhui University, Hefei, Anhui, China, Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui, China
⨯
Yazhong Xiao

* E-mail: turenzh@ahu.edu.cn (XZ); yzxiao@ahu.edu.cn (YX)

Affiliations School of Life Sciences, Anhui University, Hefei, Anhui, China, Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui, China
⨯

Structure-Based Rational Design to Enhance the Solubility and Thermostability of a Bacterial Laccase Lac15

Zemin Fang,
Peng Zhou,
Fei Chang,
Qiang Yin,
Wei Fang,
Jing Yuan,
Xuecheng Zhang,
Yazhong Xiao

Published: July 18, 2014
https://doi.org/10.1371/journal.pone.0102423

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Laccases
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Solubility
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Crystal structure
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Protein expression
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