Single-Stranded DNA Catalyzes Hybridization of PCR-Products to Microarray Capture Probes

Simon Dally; Steffen Rupp; Karin Lemuth; Stefan C. Hartmann; Ekkehard Hiller; Susanne M. Bailer; Cornelius Knabbe; Jan Weile

doi:10.1371/journal.pone.0102338

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Open Access

Peer-reviewed

Research Article

Single-Stranded DNA Catalyzes Hybridization of PCR-Products to Microarray Capture Probes

Simon Dally,

Affiliations Institute for Laboratory and Transfusion Medicine, Heart and Diabetes Center North Rhine-Westphalia, Bad Oeynhausen, Germany, Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany
⨯
Steffen Rupp,

Affiliation Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany
⨯
Karin Lemuth,

Current address: Karin Lemuth, Applied Research 1 - Microsystem Technologies - Microstructuring and Assembly, Robert Bosch GmbH, Gerlingen, Germany

Affiliation Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany
⨯
Stefan C. Hartmann,

Affiliation Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany
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Ekkehard Hiller,

Affiliation Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany
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Susanne M. Bailer,

Affiliation Department of Molecular Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany
⨯
Cornelius Knabbe,

Affiliation Institute for Laboratory and Transfusion Medicine, Heart and Diabetes Center North Rhine-Westphalia, Bad Oeynhausen, Germany
⨯
Jan Weile

* E-mail: jweile@hdz-nrw.de

Affiliation Institute for Laboratory and Transfusion Medicine, Heart and Diabetes Center North Rhine-Westphalia, Bad Oeynhausen, Germany
⨯

Single-Stranded DNA Catalyzes Hybridization of PCR-Products to Microarray Capture Probes

Simon Dally,
Steffen Rupp,
Karin Lemuth,
Stefan C. Hartmann,
Ekkehard Hiller,
Susanne M. Bailer,
Cornelius Knabbe,
Jan Weile

Published: July 15, 2014
https://doi.org/10.1371/journal.pone.0102338

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Probe hybridization
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Polymerase chain reaction
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Microarrays
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Signal amplification
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DNA hybridization
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Sense strands
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DNA isolation
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Denaturation
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