Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing

Open Access

Peer-reviewed

Research Article

Robert Wilson ,

* E-mail: R.Wilson@liv.ac.uk

Affiliation Centre for Genomic Research at the Institute of Integrative Biology, University Of Liverpool, Liverpool, United Kingdom
⨯
Christian Bourne,

Affiliation Centre for Genomic Research at the Institute of Integrative Biology, University Of Liverpool, Liverpool, United Kingdom
⨯
Roy R. Chaudhuri,

Current address: Department of Molecular Biology and Biotechnology, Sheffield University, Sheffield, United Kingdom

Affiliation Centre for Genomic Research at the Institute of Integrative Biology, University Of Liverpool, Liverpool, United Kingdom
⨯
Richard Gregory,

Affiliation Centre for Genomic Research at the Institute of Integrative Biology, University Of Liverpool, Liverpool, United Kingdom
⨯
John Kenny,

Affiliation Centre for Genomic Research at the Institute of Integrative Biology, University Of Liverpool, Liverpool, United Kingdom
⨯
Andrew Cossins

Affiliation Centre for Genomic Research at the Institute of Integrative Biology, University Of Liverpool, Liverpool, United Kingdom
⨯

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Polymerase chain reaction
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Thrombin
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Sequence motif analysis
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Next-generation sequencing
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Oligonucleotides
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DNA extraction
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Chemical dissociation
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DNA sequencing
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