An Optimized Protocol for Isolating Primary Epithelial Cell Chromatin for ChIP

Open Access

Peer-reviewed

Research Article

James A. Browne,

Affiliations Human Molecular Genetics Program, Lurie Children's Research Center, Chicago, Illinois, United States of America, Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America
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Ann Harris,

Affiliations Human Molecular Genetics Program, Lurie Children's Research Center, Chicago, Illinois, United States of America, Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America, Robert H Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America
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Shih-Hsing Leir

* E-mail: s-leir@northwestern.edu.

Affiliations Human Molecular Genetics Program, Lurie Children's Research Center, Chicago, Illinois, United States of America, Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States of America
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Chromatin
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Cell membranes
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Epithelial cells
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Lysis (medicine)
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Sonication
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Protease inhibitors
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Light microscopy
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Nuclear membrane
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