A PCR Based Protocol for Detecting Indel Mutations Induced by TALENs and CRISPR/Cas9 in Zebrafish

Open Access

Peer-reviewed

Research Article

Chuan Yu ,

Contributed equally to this work with: Chuan Yu, Yaguang Zhang

Affiliation State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and College of Life Science, Sichuan University, Chengdu, Sichuan, People’s Republic of China
⨯
Yaguang Zhang ,

Contributed equally to this work with: Chuan Yu, Yaguang Zhang

Affiliation State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and College of Life Science, Sichuan University, Chengdu, Sichuan, People’s Republic of China
⨯
Shaohua Yao ,

* E-mail: Shaohuayao@scu.edu.cn

Affiliation State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and College of Life Science, Sichuan University, Chengdu, Sichuan, People’s Republic of China
⨯
Yuquan Wei

Affiliation State Key Laboratory of Biotherapy and Cancer Center, West China Hospital and College of Life Science, Sichuan University, Chengdu, Sichuan, People’s Republic of China
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Mutation detection
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TALENs
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Zebrafish
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Polymerase chain reaction
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Point mutation
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Deletion mutation
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Gene pool
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Genomics
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