Exploring the Functional Residues in a Flavin-Binding Fluorescent Protein Using Deep Mutational Scanning

Open Access

Peer-reviewed

Research Article

HyeonSeok Shin,

Affiliation Department of Biological Sciences and KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejon, Republic of Korea
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Yoobok Cho,

Affiliation Department of Biological Sciences and KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejon, Republic of Korea
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Dong-hui Choe,

Affiliation Department of Biological Sciences and KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejon, Republic of Korea
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Yujin Jeong,

Affiliation Department of Biological Sciences and KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejon, Republic of Korea
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Suhyung Cho,

Affiliation Department of Biological Sciences and KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejon, Republic of Korea
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Sun Chang Kim,

Affiliations Department of Biological Sciences and KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejon, Republic of Korea, Intelligent Synthetic Biology Center, Daejon, Republic of Korea
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Byung-Kwan Cho

* E-mail: bcho@kaist.ac.kr

Affiliations Department of Biological Sciences and KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejon, Republic of Korea, Intelligent Synthetic Biology Center, Daejon, Republic of Korea
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Mutagenesis
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Mutation detection
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Substitution mutation
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Salt bridges
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Nucleotide sequencing
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Amino acid analysis
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Protein sequencing
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Site-directed mutagenesis
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