Selection of Reference Genes for Quantitative Real Time PCR (qPCR) Assays in Tissue from Human Ascending Aorta

Carmen Rueda-Martínez; Oscar Lamas; María José Mataró; Juan Robledo-Carmona; Gemma Sánchez-Espín; Manuel Jiménez-Navarro; Miguel Such-Martínez; Borja Fernández

doi:10.1371/journal.pone.0097449

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Peer-reviewed

Research Article

Selection of Reference Genes for Quantitative Real Time PCR (qPCR) Assays in Tissue from Human Ascending Aorta

Carmen Rueda-Martínez,

Affiliation UGC del Corazón, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Universitario Virgen de la Victoria, Málaga, Spain
⨯
Oscar Lamas,

Affiliation UGC del Corazón, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Universitario Virgen de la Victoria, Málaga, Spain
⨯
María José Mataró,

Affiliation UGC del Corazón, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Universitario Virgen de la Victoria, Málaga, Spain
⨯
Juan Robledo-Carmona,

Affiliation UGC del Corazón, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Universitario Virgen de la Victoria, Málaga, Spain
⨯
Gemma Sánchez-Espín,

Affiliation UGC del Corazón, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Universitario Virgen de la Victoria, Málaga, Spain
⨯
Manuel Jiménez-Navarro,

Affiliations UGC del Corazón, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Universitario Virgen de la Victoria, Málaga, Spain, Departamento de Medicina, Facultad de Medicina, Universidad de Málaga, Málaga, Spain
⨯
Miguel Such-Martínez,

Affiliation UGC del Corazón, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Clínico Universitario Virgen de la Victoria, Málaga, Spain
⨯
Borja Fernández

* E-mail: borjafe@uma.es

Affiliation Departamento de Biología Animal, Instituto de Investigación Biomédica de Málaga (IBIMA), Facultad de Ciencias, Universidad de Málaga, Málaga, Spain
⨯

Selection of Reference Genes for Quantitative Real Time PCR (qPCR) Assays in Tissue from Human Ascending Aorta

Carmen Rueda-Martínez,
Oscar Lamas,
María José Mataró,
Juan Robledo-Carmona,
Gemma Sánchez-Espín,
Manuel Jiménez-Navarro,
Miguel Such-Martínez,
Borja Fernández

Published: May 19, 2014
https://doi.org/10.1371/journal.pone.0097449

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Aorta
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Gene expression
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Computer software
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Aortic valve
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Algorithms
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Polymerase chain reaction
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Messenger RNA
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Reverse transcription
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