The piggyBac Transposon-Mediated Expression of SV40 T Antigen Efficiently Immortalizes Mouse Embryonic Fibroblasts (MEFs)

Ning Wang; Wenwen Zhang; Jing Cui; Hongmei Zhang; Xiang Chen; Ruidong Li; Ningning Wu; Xian Chen; Sheng Wen; Junhui Zhang; Liangjun Yin; Fang Deng; Zhan Liao; Zhonglin Zhang; Qian Zhang; Zhengjian Yan; Wei Liu; Jixing Ye; Youlin Deng; Zhongliang Wang; Min Qiao; Hue H. Luu; Rex C. Haydon; Lewis L. Shi; Houjie Liang; Tong-Chuan He

doi:10.1371/journal.pone.0097316

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Peer-reviewed

Research Article

The piggyBac Transposon-Mediated Expression of SV40 T Antigen Efficiently Immortalizes Mouse Embryonic Fibroblasts (MEFs)

Ning Wang ,

Contributed equally to this work with: Ning Wang, Wenwen Zhang

Affiliations Department of Oncology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China, Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America
⨯
Wenwen Zhang ,

Contributed equally to this work with: Ning Wang, Wenwen Zhang

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China, Department of Laboratory Medicine, the Affiliated Hospital, Binzhou Medical University, Yantai, Shandong, China
⨯
Jing Cui,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Hongmei Zhang,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Xiang Chen,

Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America
⨯
Ruidong Li,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Ningning Wu,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Xian Chen,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Sheng Wen,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Junhui Zhang,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Liangjun Yin,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Fang Deng,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Department of Cell Biology, Third Military Medical University, Chongqing, China
⨯
Zhan Liao,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Department of Orthopaedic Surgery, Xiang-Ya Hospital of Central South University, Changsha, China
⨯
Zhonglin Zhang,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Department of Surgery, Zhongnan Hospital of Wuhan University, Wuhan, China
⨯
Qian Zhang,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Zhengjian Yan,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Wei Liu,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Jixing Ye,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, School of Bioengineering, Chongqing University, Chongqing, China
⨯
Youlin Deng,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Zhongliang Wang,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Min Qiao,

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
Hue H. Luu,

Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America
⨯
Rex C. Haydon,

Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America
⨯
Lewis L. Shi,

Affiliation Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America
⨯
Houjie Liang ,

* E-mail: lianghoujie@sina.com (HL); tche@uchicago.edu (TCH)

Affiliation Department of Oncology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China
⨯
[ ... ],
Tong-Chuan He

* E-mail: lianghoujie@sina.com (HL); tche@uchicago.edu (TCH)

Affiliations Molecular Oncology Laboratory, Department of Orthopaedic Surgery, The University of Chicago Medical Center, Chicago, Illinois, United States of America, Ministry of Education Key Laboratory of Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China
⨯
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The piggyBac Transposon-Mediated Expression of SV40 T Antigen Efficiently Immortalizes Mouse Embryonic Fibroblasts (MEFs)

Ning Wang,
Wenwen Zhang,
Jing Cui,
Hongmei Zhang,
Xiang Chen,
Ruidong Li,
Ningning Wu,
Xian Chen,
Sheng Wen,
Junhui Zhang

Published: May 20, 2014
https://doi.org/10.1371/journal.pone.0097316

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