Improving the Secretion of a Methyl Parathion Hydrolase in Pichia pastoris by Modifying Its N-Terminal Sequence

Open Access

Peer-reviewed

Research Article

Ping Wang ,

Contributed equally to this work with: Ping Wang, Lu Huang

Affiliation Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
⨯
Lu Huang ,

Contributed equally to this work with: Ping Wang, Lu Huang

Affiliation Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
⨯
Hu Jiang,

Affiliation Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
⨯
Jian Tian ,

* E-mail: tianjian@caas.cn

Affiliation Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
⨯
Xiaoyu Chu,

Affiliation Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
⨯
Ningfeng Wu

Affiliation Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, P. R. China
⨯

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Protein secretion
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Pichia pastoris
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Plasmid construction
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Protein structure
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Recombinant proteins
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Yeast
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Polymerase chain reaction
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Protein expression
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