Co-Expression of Foreign Proteins Tethered to HIV-1 Envelope Glycoprotein on the Cell Surface by Introducing an Intervening Second Membrane-Spanning Domain

Hongyun Wang; Xiao Li; Shuhei Nakane; Shujun Liu; Hirohito Ishikawa; Aikichi Iwamoto; Zene Matsuda

doi:10.1371/journal.pone.0096790

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Open Access

Peer-reviewed

Research Article

Co-Expression of Foreign Proteins Tethered to HIV-1 Envelope Glycoprotein on the Cell Surface by Introducing an Intervening Second Membrane-Spanning Domain

Hongyun Wang ,

Contributed equally to this work with: Hongyun Wang, Xiao Li

Affiliation China-Japan Joint Laboratory of Structural Virology and Immunology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
⨯
Xiao Li ,

Contributed equally to this work with: Hongyun Wang, Xiao Li

Affiliations China-Japan Joint Laboratory of Structural Virology and Immunology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China, University of Chinese Academy of Sciences, Beijing, China
⨯
Shuhei Nakane,

Affiliations China-Japan Joint Laboratory of Structural Virology and Immunology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China, Research Center for Asian Infectious Diseases, Institute of Medical Science, the University of Tokyo, Tokyo, Japan
⨯
Shujun Liu,

Affiliation China-Japan Joint Laboratory of Structural Virology and Immunology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China
⨯
Hirohito Ishikawa,

Affiliations China-Japan Joint Laboratory of Structural Virology and Immunology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China, Research Center for Asian Infectious Diseases, Institute of Medical Science, the University of Tokyo, Tokyo, Japan
⨯
Aikichi Iwamoto,

Affiliations Research Center for Asian Infectious Diseases, Institute of Medical Science, the University of Tokyo, Tokyo, Japan, Division of Infectious Diseases, Advanced Clinical Research Center, Institute of Medical Science, the University of Tokyo, Tokyo, Japan
⨯
Zene Matsuda

* E-mail: zmatsuda@ims.u-tokyo.ac.jp

Affiliations China-Japan Joint Laboratory of Structural Virology and Immunology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China, Research Center for Asian Infectious Diseases, Institute of Medical Science, the University of Tokyo, Tokyo, Japan
⨯

Co-Expression of Foreign Proteins Tethered to HIV-1 Envelope Glycoprotein on the Cell Surface by Introducing an Intervening Second Membrane-Spanning Domain

Hongyun Wang,
Xiao Li,
Shuhei Nakane,
Shujun Liu,
Hirohito Ishikawa,
Aikichi Iwamoto,
Zene Matsuda

Published: May 7, 2014
https://doi.org/10.1371/journal.pone.0096790

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