The Use of Laser Microdissection in the Identification of Suitable Reference Genes for Normalization of Quantitative Real-Time PCR in Human FFPE Epithelial Ovarian Tissue Samples

Jing Cai; Tao Li; Bangxing Huang; Henghui Cheng; Hui Ding; Weihong Dong; Man Xiao; Ling Liu; Zehua Wang

doi:10.1371/journal.pone.0095974

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Open Access

Peer-reviewed

Research Article

The Use of Laser Microdissection in the Identification of Suitable Reference Genes for Normalization of Quantitative Real-Time PCR in Human FFPE Epithelial Ovarian Tissue Samples

Jing Cai ,

Contributed equally to this work with: Jing Cai, Tao Li

Affiliation Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
⨯
Tao Li ,

Contributed equally to this work with: Jing Cai, Tao Li

Affiliation Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
⨯
Bangxing Huang,

Affiliations Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China, Department of Pathology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
⨯
Henghui Cheng,

Affiliation Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
⨯
Hui Ding,

Affiliation Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
⨯
Weihong Dong,

Affiliation Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
⨯
Man Xiao,

Affiliation Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
⨯
Ling Liu,

Affiliation Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
⨯
Zehua Wang

* E-mail: zehuawang@163.net

Affiliation Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
⨯

The Use of Laser Microdissection in the Identification of Suitable Reference Genes for Normalization of Quantitative Real-Time PCR in Human FFPE Epithelial Ovarian Tissue Samples

Jing Cai,
Tao Li,
Bangxing Huang,
Henghui Cheng,
Hui Ding,
Weihong Dong,
Man Xiao,
Ling Liu,
Zehua Wang

Published: April 28, 2014
https://doi.org/10.1371/journal.pone.0095974

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Ovarian cancer
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Computer software
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Gene expression
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Uterine cancer
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Ribosomal RNA
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Epithelial cells
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