Whole-Mount MeFISH: A Novel Technique for Simultaneous Visualization of Specific DNA Methylation and Protein/RNA Expression

Open Access

Peer-reviewed

Research Article

Hirosuke Shiura,

Affiliation Technology & Development Team for Mammalian Genome Dynamics, RIKEN BioResource Center, Ibaraki, Japan
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Akimitsu Okamoto,

Affiliation Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan
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Hiroyuki Sasaki,

Affiliation Division of Epigenomics and Development, Medical Institute of Bioregulation, and Epigenome Network Research Center, Kyushu University, Fukuoka, Japan
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Kuniya Abe

* E-mail: abe@rtc.riken.jp

Affiliations Technology & Development Team for Mammalian Genome Dynamics, RIKEN BioResource Center, Ibaraki, Japan, Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan
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DNA methylation
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Immunostaining
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RNA hybridization
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Embryos
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Probe hybridization
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Epigenetics
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DNA denaturation
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Signal amplification
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