Rapid and Efficient Isolation of High-Quality Small RNAs from Recalcitrant Plant Species Rich in Polyphenols and Polysaccharides

Jun Peng; Zihao Xia; Ling Chen; Minjing Shi; Jinji Pu; Jianrong Guo; Zaifeng Fan

doi:10.1371/journal.pone.0095687

Loading metrics

Open Access

Peer-reviewed

Research Article

Rapid and Efficient Isolation of High-Quality Small RNAs from Recalcitrant Plant Species Rich in Polyphenols and Polysaccharides

Jun Peng ,

Contributed equally to this work with: Jun Peng, Zihao Xia, Ling Chen, Minjing Shi

Affiliations State Key Laboratory of Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing, China, Ministry of Agriculture Key Laboratory of Integrated Pest Management on Tropical Crops, Environmental and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China
⨯
Zihao Xia ,

Contributed equally to this work with: Jun Peng, Zihao Xia, Ling Chen, Minjing Shi

Affiliation State Key Laboratory of Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing, China
⨯
Ling Chen ,

Contributed equally to this work with: Jun Peng, Zihao Xia, Ling Chen, Minjing Shi

Affiliation State Key Laboratory of Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing, China
⨯
Minjing Shi ,

Contributed equally to this work with: Jun Peng, Zihao Xia, Ling Chen, Minjing Shi

Affiliation Ministry of Agriculture Key Laboratory for Rubber Biology, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, China
⨯
Jinji Pu,

Affiliation Ministry of Agriculture Key Laboratory of Integrated Pest Management on Tropical Crops, Environmental and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China
⨯
Jianrong Guo,

Affiliation Ministry of Agriculture Key Laboratory of Integrated Pest Management on Tropical Crops, Environmental and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China
⨯
Zaifeng Fan

* E-mail: fanzf@cau.edu.cn

Affiliation State Key Laboratory of Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing, China
⨯

Rapid and Efficient Isolation of High-Quality Small RNAs from Recalcitrant Plant Species Rich in Polyphenols and Polysaccharides

Jun Peng,
Zihao Xia,
Ling Chen,
Minjing Shi,
Jinji Pu,
Jianrong Guo,
Zaifeng Fan

Published: May 1, 2014
https://doi.org/10.1371/journal.pone.0095687

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Small interfering RNA
Is the Subject Area "Small interfering RNA" applicable to this article?

Thanks for your feedback.
RNA extraction
Is the Subject Area "RNA extraction" applicable to this article?

Thanks for your feedback.
MicroRNAs
Is the Subject Area "MicroRNAs" applicable to this article?

Thanks for your feedback.
Northern blot
Is the Subject Area "Northern blot" applicable to this article?

Thanks for your feedback.
RNA hybridization
Is the Subject Area "RNA hybridization" applicable to this article?

Thanks for your feedback.
Plant tissues
Is the Subject Area "Plant tissues" applicable to this article?

Thanks for your feedback.
Plants
Is the Subject Area "Plants" applicable to this article?

Thanks for your feedback.
Polysaccharides
Is the Subject Area "Polysaccharides" applicable to this article?

Thanks for your feedback.