A Modified Screening System for Loss-of-Function and Dominant Negative Alleles of Essential MCMV Genes

Open Access

Peer-reviewed

Research Article

Madlen Pogoda,

Affiliations Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität, Munich, Germany, DZIF - German Center for Infection Research, Munich, Germany
⨯
Jens B. Bosse,

Current address: Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America

Affiliation Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität, Munich, Germany
⨯
Karl-Klaus Conzelmann,

Affiliation Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität, Munich, Germany
⨯
Ulrich H. Koszinowski,

Affiliation Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität, Munich, Germany
⨯
Zsolt Ruzsics

* E-mail: ruzsics@mvp.uni-muenchen.de

Affiliations Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität, Munich, Germany, DZIF - German Center for Infection Research, Munich, Germany
⨯

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Recombination reactions
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Transfection
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Plasmid construction
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Genetic screens
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Mutagenesis
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Recombination-based assay
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DNA recombination
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Viral genomics
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